Genetic Transformation of Sorghum for Insect-Pest Resistance

Sorghum is a major food and fodder crop for dry land farmers in India and elsewhere. One of the major reasons for lower yields in this crop is due to the problem of insects, pests such as stem borer and shoot fly in the major sorghum growing parts of India.

To develop the insect pest resistant transgenic plants, we are using Agrobacterium mediated genetic transformation using binary vector pCAMBIA 3300. This vector contains nptII gene, which codes kanamycin for bacterial selection, while having bar gene, which codes basta for plant selection, and also having Cry1Aa gene for insect resistance.

Genetic transformation was carried out in two different Sorghum genotypes viz., NTJ-2 and ICSB-38 using different media, for multiple shoot (MS) induction (BAP, NAA), maturation (BAP), and germination (BAP, IBA). Eywords Agrobacterium. nptII. Bar gene. PCAMBIA 3300. Basta. NTJ-2 and ICSB-38. Kanamycin. C58 Agrobacterium strain.

 

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